【摘要 】 目的 建立慢性間歇低氧幼鼠模型 ,研究其空間學(xué)習(xí)記憶能力和海馬 CA1區(qū)神經(jīng)元超微結(jié)
構(gòu)的變化。方法 八臂迷宮訓(xùn)練成功的 3～4周齡 S D幼鼠 40只隨機(jī)分 2周間歇低氧組 ( 2H)、 對(duì)照組
(2C)和 4周間歇低氧組 (4H)、 對(duì)照組 ( 4C) ,建立慢性間歇低氧動(dòng)物模型 ,間歇低氧結(jié)束后八臂迷宮測(cè)試
學(xué)習(xí)記憶能力 ,透射電鏡觀察海馬 CA1區(qū)神經(jīng)元的超微結(jié)構(gòu)變化。結(jié)果 間歇低氧組參考記憶錯(cuò)誤
[ (3 . 30 ± 1 . 06)次、 (2 . 00 ± 0 . 67)次 ]、 工作記憶錯(cuò)誤 [ (2 . 30 ± 0 . 67)次、( 1 . 00 ± 0 . 82)次 ]和總錯(cuò)誤次數(shù)
[ (5 . 40 ± 1 . 78)次、 (3 . 00 ± 0 . 82)次 ]均高于對(duì)照組 ( P < 0 . 05) ,且隨著間歇低氧時(shí)間的延長(zhǎng) ,這種錯(cuò)誤次
數(shù)增多 ( P < 0 . 05) ;電鏡下間歇低氧組海馬 CA1區(qū)神經(jīng)細(xì)胞的形態(tài)、 胞漿內(nèi)的細(xì)胞器均有明顯變化。結(jié)論
慢性間歇低氧可引起幼鼠空間學(xué)習(xí)記憶能力下降 ,這可能和海馬 CA1區(qū)神經(jīng)元超微結(jié)構(gòu)改變有關(guān)。
【關(guān)鍵詞 】 慢性間歇低氧; 模型; 記憶; 海馬; 超微結(jié)構(gòu)
Establ ishmen t of i n term itten t hypox i a model i n wean l i ng ra ts and its effects on the ab il iti es of learn i ng and
memory ZHOU Yong2 hai , CA I X iao2 hong, Zhang Cun2 xue, et al . Depart m ent of Respiratory M edicine Yuy ing
Children’ s Hospital Aff iliated toW enzhou M edical College, W enzhou 325027, China
【Abstract】 Objecti ve To establish a model of inter mittent hypoxia in weanling rats and study the change
of spatial learning and memory abilities and ultrastructure of hi ppocampal CAl neur ons . Methods Forty 32 week2
old～42 week2 old Sp rague Dawley rats which have been trained t o comp lete the 82 Arm (42 ar m baited) radialmaze
were randomly divided int o 22 week2 hypoxia gr oup ( 2H) , 42 week2 hypoxia group ( 4H) , 22 week2 contr ol gr oup ( 2C)
and 42 week2 control group (4C) . I nter mittent hypoxia modelwas induced by an inter mittent hypoxia cabin . At the
end of study, 82 Arm (42 ar m baited) radialmaze was used t o measure spatialmemory in these weanling rats and the
ultrastructure of hi ppocampal CAl neur onswas studied by electr on micr oscope . Results The nadir of ambient oxy2
gen concentrati on was about 7 . 8% and the normal oxygen concentrati on was about 21 . 0%. Both 4H and 2H rats
had significantlymore err ors choices than 4C and 2C rats( P < 0 . 05, respectively) . More RME,WME and TE were
found in 4H gr oup (3 . 30 ± 1 . 06, 2 . 30 ± 0 . 67, 5 . 40 ± 1 . 78) than in 2H gr oup (2 . 00 ± 0 . 67, 1 . 00 ± 0 . 82, 3 . 00
± 0 . 82) ( P < 0 . 05) . The nuclearmembrane and mit ochondria of hi ppocampal CAl neur ons in inter mittent hypox2
ia group s were dist orted compared with those in control gr oup s . Conclusi on Chronic inter mittent hypoxia could
result in damage and lesi on of spatial learning and memory in weanling rats . Itmay be correlated with the changes
of hi ppocampal CA1 neurons .