[摘要 ] 目的 　 評價(jià)天麻醒腦膠囊對動物學(xué)習(xí)記憶功能的影響及其初步作用機(jī)理 1方法 　采用東莨菪堿、
乙醇及亞硝酸鈉復(fù)制小鼠和大鼠記憶障礙模型 , 運(yùn)用避暗法研究天麻醒腦膠囊對動物學(xué)習(xí)記憶功能的影響 ,
同時(shí)探討其對腦組織乙酰膽堿酯酶 (AChE) 活性、丙二醛 (MDA) 及脂褐質(zhì)水平的影響 1結(jié)果 　高、中劑量
(大鼠: 高劑量 112 g/kg, 中劑量 016 g/kg; 小鼠: 高劑量 214 g/kg, 中劑量 112 g/kg) 的天麻醒腦膠囊能明顯
減少給藥組大鼠及小鼠 5min內(nèi)的錯(cuò)誤次數(shù) , 延長觸電潛伏期 1高、中、低 3個(gè)劑量的天麻醒腦膠囊均可減少
大鼠腦組織 MDA水平 , 降低 AChE活性 , 同時(shí)明顯抑制腦組織脂褐質(zhì)含量的升高 1結(jié)論 　天麻醒腦膠囊通過
多種途徑改善記憶損傷模型動物的學(xué)習(xí)記憶功能 , 其機(jī)制可能與降低腦組織 AChE活性 , 降低腦組織中脂質(zhì)過
氧化物、脂褐質(zhì)等的含量有關(guān) 1提示天麻醒腦膠囊具有良好的促智作用 1
[關(guān)鍵詞 ] 天麻醒腦膠囊; 避暗法; 學(xué)習(xí)記憶; 丙二醛; 脂褐質(zhì); 乙酰膽堿酯酶
Effects of Ti maxi ngnao Capsule on the Abi liti es of Learn i ng and
Memory and Its Preli m i naryMechan is ms
CHEN Peng
1)
, J I N Yan
2)
, WANGDian - hua
1)
, ZHEN J in - dan
3)
, ZHANG J ia - shuo
1)
, SHEN Zhi - qiang
1)
( 1) Yunnan Phar m acological Labora tories of N atu ral Products, Kunm ing M edical College, Kunm ing 650031;
2) Dept . of M edicam ent , People’ s Hospital in Yunnan, Kunm ing 650011;
3) Yong Z itang Phar m aceutical L td Co of Yunnan Province, Kunm ing 650021, China)
[Abstract ] O b j e c tive　To study the effects of Ti maxingnao Cap sule on the abilities of learning and memo2
ry and its p reli minary mechanis ms1Me tho d s　I CR mice and SD rats were res pectively induced by scopolamine
hydrobromide, alcohol, and NaNO2 t o make models1The test of avoidance res ponse t o darkness was used1The
influence of Ti maxingnao Cap sule was investigated on acetylcholine esterase (AChE) , mal ondiadehyde (MDA)
and li pofuscin in brain tissue1Re su lts　I n the test of avoidance res ponse t o darkness, Tianmaxingnao Cap sule
(112 and 214 g/kg formice, 016 and 112 g/kg for rats) could significantly reduce the err or ti meswithin 5min
in dys mnesia gr oup s ( P < 0101, P < 0105) and p rol ong the incubati on peri ods ofmemory1The three doses of
Tianmaxingnao Cap sule decreased the level ofMDA, supp ressed the activity ofAChE and obvi ously inhibited the
increase of the li pofuscin in rat brain tissue1Co nc lu s i o n　Tianmaxingnao Cap sule could i mp rove the abilities of
learning and memory in ani malmodels thr ough multi p le ways1The p reli minarymechanis msmay be ass ociated withits effects on decreasing the activity of AChE, p romoting the functi on of cerebral monoamine nervous system in
brain tissue and reducing the level of li p id peroxidati on in brain tissue1 It is suggested that Tianmaxingnao Cap sule
have the effects of i mp r oving learning and memory1
[ Key words] Tianmaxingnao cap sule; Avoidance res ponse t o darkness ; Learning and memory; Mal ondia2
dehyde; Li pofuscin; Acetylcholine esterase